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KMID : 0380919960250020294
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Journal of the Korean Society of Food Nutrition
1996 Volume.25 No. 2 p.294 ~ p.300
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Characterization of Red - Pigment Produced by Marine Bacterium Vibrio sp.
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Kim Hak-Ju
Park Hyo-Jin
Bae Seoung-Kwon
Kim Jong-Deog
Kong In-Soo
Kong Jai-Yul
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Abstract
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A marine bacterium producing red-pigment was isolated from southern sea of Korea and was identified as Vibrio sp.. The strain was growing well on LB culture medium and red-pigment was accumulated intracellularly. The productivity of red-pigment reached the maximum value after 24 hours and increased with addition of 1% fructose or 0.3% (NH₄)₂SO₄. The extracted red-pigment from the cell showed the highest absorbance at 531㎜ by UV-VIS spectrophotometer and the value of wavelength(λ=531㎜) was similar to that of anthocyan group. Using thin layer chromatography, red-pigment was separated into two different components ; in addition, the purity of these components were analyzed by HPLC. Molecular weight of these components were 281 and 236, respectively. Therefore, the red pigment from marine bacterium Vibrio sp. was most likely to be composed mainly of cyanidin(M.W.=281).
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KEYWORD
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marine bacterium, vibrio sp., red-pigment, anthocyan
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